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DRX486699: Deep-sequencing of SAMD00642679
1 ILLUMINA (Illumina MiSeq) run: 605,572 spots, 170.6M bases, 104.6Mb downloads

Submitted by: KUMAMOTO
Study: Vulnerability to APOBEC3G linked to the pathogenicity of deltaretroviruses
show Abstracthide Abstract
Mutational analysis of provirus using samples from HTLV-1 carriers, HTLV-2-infected individuals, and STLV-1-infected Japanese macaques. Further, RNA-seq and ChIP-seq demonstrated that activation of TGF-beta is important for ATL.
Sample: HTLV-1_S21
SAMD00642679 • DRS347807 • All experiments • All runs
Library:
Name: HTLV-1_carrier_S21
Instrument: Illumina MiSeq
Strategy: Targeted-Capture
Source: GENOMIC
Selection: size fractionation
Layout: PAIRED
Construction protocol: Genomic DNA from HTLV-1 carriers, HTLV-2 infected individuals, and STLV-1 infected Japanese macaques was extracted with phenol-chloroform. To amplify the whole provirus sequences of HTLV-1, HTLV-2, and STLV-1, long-PCR was performed. The libraries were prepared using the Nextera XT DNA Library Preparation Kit (Illumina), according to the manufacturer's instructions. Then deep-sequencing analysis was performed with MiSeq (Illumina).
Pipeline: show...hide...
StepPrev Step
1NIL
Runs: 1 run, 605,572 spots, 170.6M bases, 104.6Mb
Run# of Spots# of BasesSizePublished
DRR502704605,572170.6M104.6Mb2023-12-31

ID:
31150224

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